Endocrine Fact Sheet No. 3.1 from Axiom Veterinary Laboratory

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ENDOCRINE FACT SHEET No. 3.1
 Diagnosis of "Equivocal" Hyperthyroidism 
by
Dr. Richard Dixon
BVMS, PhD, CertVR, MRCVS.
Factsheet  Title

 1.0
Introduction to Canine Hypothyroidism

 1.1
Therapy and therapeutic monitoring of Canine Hypothyroidism

 2.0
Introduction to Canine Hyperadrenocorticism

 2.1
Trilostane Treatment in Canine Cushing's Syndrome

 3.0
Introduction to Canine Hyperadrenocorticism

 3.1
Diagnosisof "Equivocal" Hyperthyroidism

 3.2
Managing Post-Operative Hypocalcaemia

 4.0
Introduction to Canine Diabetes Mellitus

 4.1
Stabilising the Healthy Diabetic Dog

 4.2
"The Difficult Diabetic"

 5.0
Introduction to Feline Diabetes Mellitus

 5.1
Stabilising the Healthy Diabetic Cat

 6.0
Introduction to Insulinomas

 7.0
Introduction to Feline Acromegaly

 8.0
Introduction to Canine Hypoadrenocorticism

 8.1
Emergency therapy for dogs with an acute hypoadrenocortical (Addisonian) crisis
  Some Common Small Animal Endocrine Test Protocols 

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  Diagnosis of "Equivocal" Hyperthyroidism 

Background
The diagnosis of hyperthyroidism can be confirmed in the majority of cats based on the presence of appropriate clinical signs and an increased circulating total T4 concentration. At Axiom, a total T4 value > 60nmol/L is considered supportive of hyperthyroidism. However, the upper end of the reference range in cats extends to 40nmol/L. Those cases with total T4 values between 40-60 nmol/L are therefore in the equivocal range. There are several possible explanations for equivocal results. The most common explanations are either
1. very early hyperthyroidism
2. early hyperthyroidism with concurrent nonthyroidal illness
3. random fluctuation into the equivocal range in a euthyroid cat
Various diagnostic options are available in clarifying the thyroid status of cats with equivocal results.

"Evaluation in Context"
As in dogs, total T4 concentrations generally decrease in cats with significant nonthyroidal illness. Thus most cats with obvious or chronic clinical abnormalities are expected to have subnormal or low-normal values. The presence of an equivocal result in these cases should be considered as inappropriate and increase the suspicion of early hyperthyroidism (with/without concurrent nonthyroidal illness). Certainly an equivocal result in a cat that looks clinically severely ill is highly suspicious of early hyperthyroidism with concurrent illness.

Wait and Retest
In otherwise relatively healthy cats it is often appropriate to wait for 4-6 weeks and re-test total T4 concentrations. If euthyroid, it is unlikely that a second sample will be within the equivocal range. However, if hyperthyroid it is quite likely that the result will have increased into the diagnostic range. This approach is only appropriate for relatively clinically healthy cats.

Free T4 Measurement
Free T4 estimation is generally considered to be a slightly more diagnostically "sensitive" test for hyperthyroidism. i.e. it is slightly more likely to give a positive result in early cases than is total T4 measurement. Free T4 measurement may therefore be a useful second line test in equivocal cases. However, note that some cats with nonthyroidal illness will have temporary increases in free T4 and so this is not routinely recommended as the sole test in routine investigation of feline hyperthyroidism

T3 Suppression Test
Administration of exogenous T3 to a euthyroid cat causes an increased negative feedback effect on TSH secretion from the pituitary gland and consequently circulating total T4 concentrations decrease. However, cats with hyperthyroidism secrete T4 independent of TSH control and so T3 administration has little effect on total T4 values in these cats. This test can therefore be used to evaluate "equivocal" hyperthyroidism.
Test Protocol: Collect blood for total T4 measurement, administer 0.02 mg/kg T3 (Tertroxin) orally, TID for 7 doses; collect blood 2-4 hours after the last dose of T3 for total T4 and total T3 measurement. Total T4 values should decrease to < 20nmol/L with >50 % suppression compared to the baseline value to indicate euthyroidism. Failure to suppress is consistent with hyperthyroidism. The T3 measurement is performed solely to confirm adequate owner compliance in medicating with T3. 

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