Case Study 03 - Respiratory tract infection caused by possible Cryptococcus sp. and C.aerophilia

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Case Study - 3 - Discussion
by Beverley Highfield BSc. (Hons). St. Helens Laboratory Manager

The anatomic components of the lower respiratory tract include the trachea, bronchi, bronchioles and alveoli. The trachea extends from the base of the larynx to the carina and is composed of incomplete cartilaginous rings supported by connective tissue and smooth muscle lined by cilated epithelium.

Smaller airways or bronchioles have no cartilaginous support, are composed of smooth muscle and are lined by cilated and non-cilated cuboidal epithelium (Raskin et al, 2001), cells of which are present in the wash cytology of this animal.

A bronchoalveolar lavage (BAL) is used to sample the smaller airways and alveoli of the lower respiratory tract. It is conducted by pacing a small fiberoptic scope into the lung of a patient and injecting sterile saline which is then removed. The sterile solution removed contains secretions, cells and protein from the lower respiratory tract. The sample is then analysed for possible disease processes occurring in the lung. BAL is an easy and inexpensive test which provides a high diagnostic yield. The samples can be used for cytological examination of airway disease as well as for culture analysis, but it is important to obtain a sample in a manner that will give a large number of well preserved cells.

The findings as a result of the BAL in this case are cilated epithelial cells and the organisms, Cryptococcus neoformans and Capillaria aerophilia.

In domestic pets Cryptococcus neoformans is the causative agent of the disease Cryptococcosis. The organism is found in cytological specimens as a round to oval yeast form measuring approximately 4-10 µm in diameter. C.neoformans is a common fungal agent causing chronic upper respiratory tract disease primarily in cats, and inhalation of the aerosolised organism is the most probable source of infection, however the exact mode of transmission is unknown (Raskin et al, 2001). The average age of infected cats is 5 years and there is a predilection for infection in outdoor males, most likely due to the increased roaming of tom cats (Jacobs et al, 1997).


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The organism can deposit in the upper or lower respiratory tract. However, the organism primarily remains in the upper airways since the encapsulated organisms are larger than the diameter of the terminal airways. This leads to a predominance of upper respiratory tract infections rather than pulmonary disease (Gerds-Grogen et al, 1997).

Mammals with normal immune systems usually clear the cryptococcal infection prior to its spread. The majority of resistance is provided by cell-mediated immunity. Infections with C.neoformans may indicate immunosuppression - damage to the immune system which has left the animal vulnerable to a wide variety of diseases. Immunosuppression is readily seen in cats infected with feline leukaemia virus (FeLV) or feline immunodeficiency virus (FIV). It is this reason that it has been suggested that this cat's FeLV/FIV status be checked.

The clinical signs of infection include respiratory infections (seen in >80% of cases), characterised by sneezing, nasal discharge and respiratory distress (Gerds-Grogan et al, 1997). Although these signs are presented in this case a definitive diagnosis may require further testing. The apparent encapsulated organism may be C.neoformans but two similar organisms Histoplasma capsulatum and Blastomyces dermatitis have been reported in upper respiratory infections, but are rare.
It would be recommended to use methods of immunohistochemistry to detect the presence of specific proteins in these cells and to lead to a more accurate identification of crytococcosis.

A study by Legendre & Toal in 2000 suggested that the next logical choice for diagnostic testing is latex agglutination, which detects the crytococcal capsular antigen of all known serotypes found in serum, urine or CSF. The specificity of this test is highest when the serum is pre-treated with a protease (pronase). The latex agglutination test is useful for diagnostic purposes and for the monitoring of treatment to Cryptococcosis. Further diagnosis involves immunostaining in tissue biopsies, complement fixation or fungal culture (Raskin et al, 2001).

Infection with Capillaria aerophilia is diagnosed by finding the adult nematodes or characteristic ova in nasal secretions. One egg was found in the wash cytology of the cat in this case.
C.aerophilia in cats has a direct cycle, with infected eggs being ingested with food or water. The pathogenic effects of these lungworms depend on their location within the respiratory tract, the number of infective larvae ingested and the animal's immune state.
Clinical signs of lungworm infection range from moderate coughing with slight increased respiratory rates to severe persistent coughing and respiratory distress, even failure (Raskin et al, 2001).

References

Gerds-Grogan S & Dayrell-Hart B: Feline Cryptococcosis: A retrospective evaluation. J Am Anim Hosp Assoc 33:118-122, 1997.

Jacobs GJ, Medleau L, Calvert C & Brown J: Cryptococcal infection in cats: Factors influencing treatment outcome, and results of sequential serum antigen titers in 35 cats. J Vet Int Med 11(1):1-4, 1997.

Legendre AM, Toal RL: Diagnosis and treatment of fungal diseases of the respiratory system.WB Saunders, Philadelphia, 2000, pp 815-819.

Raskin, RE & Mayer, DJ (2001). Atlas of Canine and Feline Cytology. W.B. Saunders Company.


CASE STUDIES by
Beverley Highfield
BSc. (Hons).
St. Helens Lab Manager  
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Respiratory tract infection caused by possible Cryptococcus sp. and C.aerophilia
CASE STUDY by
Yvonne McGrotty
BVMS, CertSAM, DipECVIM
-CA, MRCVS.
 
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